Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice
نویسندگان
چکیده
منابع مشابه
Two-photon voltage imaging using a genetically encoded voltage indicator
Voltage-sensitive fluorescent proteins (VSFPs) are a family of genetically-encoded voltage indicators (GEVIs) reporting membrane voltage fluctuation from genetically-targeted cells in cell cultures to whole brains in awake mice as demonstrated earlier using 1-photon (1P) fluorescence excitation imaging. However, in-vivo 1P imaging captures optical signals only from superficial layers and does n...
متن کاملSparse odor coding in awake behaving mice.
Responses of mitral cells represent the results of the first stage of odor processing in the olfactory bulb. Most of our knowledge about mitral cell activity has been obtained from recordings in anesthetized animals. We compared odor-elicited changes in firing rate of mitral cells in awake behaving mice and in anesthetized mice. We show that odor-elicited changes in mitral cell firing rate were...
متن کاملNeurovascular Coupling Studies in Awake-behaving Mice
The mechanism regulating cerebral blood fl ow (CBF) during brain function (neurovascular coupling) has been widely investigated in animals under anesthetized conditions, though anesthesia is known to greatly aff ect neurovascular physiology. The present study aims to develop a novel model for neurovascular coupling studies in awake-behaving mice. Male C57BL/6J mice were initially anesthetized w...
متن کاملUltrafast pulse compression by semiconductor two-photon gain.
We demonstrate experimentally the compression of femtosecond-scale pulses by two-photon gain in a compact electrically driven AlGaAs waveguide. Dynamic control of the pulse width from 240 to 140 fs is achieved by varying the current injection levels--in good agreement with the calculations. The pulse width is measured by a high-sensitivity intensity autocorrelator based on two-photon absorption...
متن کاملLong-Term Two-Photon Imaging in Awake Macaque Monkey
Successful application of two-photon imaging with genetic tools in awake macaque monkeys will enable fundamental advances in our understanding of higher cognitive function at the level of molecular and neuronal circuits. Here we report techniques for long-term two-photon imaging in awake macaque monkeys. Using genetically encoded indicators including GCaMP5 and GCaMP6s delivered by AAV2/1 into ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell
سال: 2019
ISSN: 0092-8674
DOI: 10.1016/j.cell.2019.11.004